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Ateh1/pan1

WebAug 13, 2024 · In Arabidopsis, AtEH/Pan1 proteins play a dual role, where on one hand, they drive actin-mediated autophagy, and on the other hand, they bind auxiliary endocytic adaptors as well as the plasma membrane (Wang et al., 2024; Yperman et al., 2024). WebMar 17, 2024 · Here we used live-cell imaging and biochemical approaches to investigate the function of the Arabidopsis thaliana TPC subunit AtEH1/Pan1. Using in vitro and in …

Articles in 2024 Nature Communications

WebJan 28, 2024 · Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion... WebThe Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are … black pearl software factory https://bdmi-ce.com

RCSB PDB - 6YET: Second EH domain of AtEH1/Pan1

WebMar 18, 2024 · Clathrin-mediated endocytosis (CME) is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form … WebSep 1, 2024 · AtEH1/Pan1 contains a typical VCA motif, which is responsible for ARP2/3 activation; it is able to bind to actin filaments and interact with APR2/3 subunits, suggesting a similar NPF function in plants. AtEH/Pan1 localizes to autophagosomes and promotes endosome to autophagosome conversion (Figure 2 B). WebIn particular, two TPC members, AtEH1/Pan1 and AtEH2/Pan1 play an important role in this pathway (Figure 8) and several components of the endocytic machinery are associated with this pathway, either as machinery or as cargo. Ongoing research focusses on unravelling in detail how this pathway is initiated, as well as how it is connected to the ... black pearl solar company

wwPDB: 6YIG

Category:Figure 3: The first EH domain of AtEH1/Pan1 interacts …

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Ateh1/pan1

The TPLATE subunit is essential for structural assembly of

WebBefore cross-linking, TML and AtEH1/Pan1 subunits were expressed in Arabidopsis cell cultures grown in continous dark (Plant Systems Biology-Dark; PSB-D) with a C-terminal … WebDec 5, 2024 · The authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles. 16 PDF View 2 excerpts, cites background A historical perspective on the regulation of cellulose …

Ateh1/pan1

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WebMar 17, 2024 · Our results reveal that AtEH/Pan1 proteins act as scaffolds to direct endocytic machinery to specific plasma membrane regions to initiate internalisation. These findings provide new insight into the interplay between membranes and protein condensates. Competing Interest Statement The authors have declared no competing … WebNov 13, 2024 · AtEH1/Pan1 and AtEH2/Pan1 are essential for plant life. In AtEH1/Pan1 heterozygous mutants, shrivelled and normal looking pollen grains are observed in a …

WebApr 1, 2024 · Title: Crystal structure of the N-terminal EF-hand domain of Arabidopsis thaliana AtEH1/Pan1 DOI: 10.2210/pdb6yig/pdb Primary publication DOI: 10.1038/s41467-021-23314-6 WebThe first EH domain of AtEH1/Pan1 interacts with the N-terminal double NPF motif of SCAMP5. a, Graphical representation of SCAMP5, the double N-terminal NPF motif is …

WebApr 23, 2024 · Collectively, our structural and functional studies reveal distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connect the internalization of SCAMP5 to the TPLATE complex. AtEH/Pan1 proteins contain two N-terminal Eps15 homology (EH) domains and are subunits of the endocytic TPLATE … WebMar 18, 2024 · The authors combine X-ray crystallography, NMR and MD simulations with biochemical and in planta analysis to characterize the two AtEH1/Pan1 EH domains and reveal their structural differences and complementary functional roles. Expand 16 Highly Influential PDF View 24 excerpts, references background, methods and results ... 1 2 3 4 …

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WebDec 14, 2024 · The two EH domains of atEH1, EH1.1 and EH1.2 as defined by ref. 21, were amplified from synthetic AtEH1/Pan1 (codon optimized for bacterial expression, IDT) (SI … black pearls on fjordurWebMar 25, 2024 · Collectively, our structural and functional studies reveal distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connect the … garfield produceWebThe TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and … garfield produce chicagoWebMar 17, 2024 · AtEH1/Pan1 and AtEH2/Pan1 are plant-specific subunits of TPC that are connected to the other six subunits via a physical interaction with the TML subunit … garfield produce companyWebYeast transformed with pGBKT7-S7-2/pGADT7 or pGBKT7/pGADT7-SKP1 Maize used as negative controls were unable to grow. Techniques Used: Positive Control, Sequencing, Construct, Transformation Assay control plasmids pgbkt7 p53 ( TaKaRa ) TaKaRa is a verified supplier TaKaRa manufactures this product About News Press Release Team … black pearls on extinctionWebApr 9, 2024 · The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endo-cytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homo-logues of the yeast ARP2/3 ... black pearls on gen 2black pearls on aberration