WebAccording to CTB staining, PDMP treatment efficiently reduced GM1 ganglioside synthesis in JHH7 cells (Figure 3A). In a 3D culture system as a mimic of the tumor microenvironment enriched with CSC-like cells , PDMP treatment inhibited the spheroid proliferation of JHH7 cells in a dose- and time-dependent manner (Figure 3B). These data are in ... WebApr 15, 2012 · In the population expressing GFP–Myo1c (Fig. 3A, see the cells marked with arrowheads), however, the percentage of cells with an intense CTB staining more than doubled (from ~20% to ~50%). To confirm these observations, a HeLa cell line stably expressing homogenous levels of GFP–Myo1c was created using FACS sorting ( Fig. 3B ).
Portions of SCCs are almost sealed. J774 cells were incubated with ...
WebAn optimized staining procedure is required to stain lipid rafts exclusively on the cell surface. Unstimulated T cells show a few CTB binding spots on the cell surface. The size … WebDec 7, 2002 · Indeed, in addition to punctate staining with CTB and the Lyn24-GFP fusion protein, both of these reagents exhibited a weak generalized membrane staining, which we presume indicates the … grafton nd school district
Association of polybrominated diphenyl ether (PBDE) levels with ...
WebCholera Toxin B subunit (CTB); FITC conjugate, lyophilized powder; This compound is a featured product for Cyclic Nucleotide research; The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport; The cholera tox WebJul 16, 2009 · Historically, the use of the neuroanatomical tracer cholera toxin subunit B (CTB) has been limited to single-labeling techniques using bright-field horseradish peroxidase and DAB staining 1,2,3,4 ... WebNov 19, 2024 · Clustering of lipid rafts containing GM1 gangliosides at the cleavage furrow is crucial for CAR formation between the nascent reticulocyte and pyrenocyte border. 45 First, we studied CTB distribution using immunofluorescence microscopy and observed that, in control erythroblasts, CTB staining was strong and more intense around the nucleus, at ... grafton nd police department