H2ax fitc
WebThe FITC signal was measured in isolated cells using flow cytometry. It was found that the gamma-H2AX signal only increased 2–3-fold in CLM-treated WBC using published protocols ( 31 ). Gamma-H2AX is a nucleosomal protein, and therefore retained in the nucleus along with other histones when cells are lysed at physiological salt concentration. Web英文名称 Rat Ultrasensitivity Thyroxine, u-T4 Elisa Kit 规格 96T/48T 品牌 研生 用途 仅供科研实验
H2ax fitc
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WebJan 3, 2024 · Distributional analysis of cell cycle phases of HDAC1 or HDAC2 knock outs compared to their control were performed by propidium iodine staining and flow cytometry measurement, respectively. b, To analyze apoptosis in HDAC1 and HDAC2 CRISPR/Cas9 knock outs, DNA double strand breaks were measured with anti-phospho-histone H2AX … WebBioLegend's FITC anti-H2A.X Phospho (Ser139) is a Mouse monoclonal antibody. The FITC anti-H2A.X Phospho (Ser139) Antibody was generated using H2A.X variant histone as the antigen. It reacts with Human, and Mouse. This antibody has been shown to work in applications such as: Staining, and Flow Cytometry.
WebSee all gamma H2A.X primary antibodies Description Rabbit polyclonal to gamma H2A.X (phospho S139) Host species Rabbit Specificity Using IF, this antibody was shown to … WebPhosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, …
WebJul 6, 2015 · We stained MEFs with a γ-H2AX specific antibody conjugated to FITC and used imaging flow cytometry to quantitate the number of γ-H2AX positive foci. In these experiments the number of cells containing 0–10 γ -H2AX positive foci ( i . e ., using a spot count algorithm) were calculated for both wt and Alkbh8 -/- MEFs for 20,000 cells, with ... WebGamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. The kit provides sufficient reagents for up to 100 stainings in 96- well plate.
WebFeb 1, 2015 · Total and phosphorylated forms of H2AX were determined by labeling cells with either anti–histone H2AX-FITC or anti–phospho–histone H2AX-PerCP (FlowCellect Dual Detection kits; Millipore, Billerica, MA) for 30 minutes in the dark at room temperature.
WebJul 5, 2007 · Cells were rinsed and suspended in FITC-conjugated anti-mouse IgG antibody diluted 1:20 in HBT for 45 min in the dark. Cells were then rinsed and suspended in 1× propidium iodide containing RNase at 4°C and quantified for FITC (γH2AX) and propidium iodide (DNA content) using a Coulter Elite ESP cell sorter. Results palissade persienne boisWebJun 27, 2006 · H2AX phosphorylation after exposure to ionizing radiation can be inhibited by the kinase inhibitor wortmannin (Fig. 5d). ... The assay was carried out according to the manufacturer's instructions with one major modification: the FITC-labeled antibody was incubated overnight at 4°C instead of 20 min on ice. palissade pas cher brico dépôtWebA431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures. Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug. Secondary antibody Mouse IgG-Fc (FITC), ab97264 , 1mg. palissade persienneWebJun 11, 2012 · The number of platelets in blood was increased significantly 7d after wounding to 1765 ± 167 x 10 3 cells/μl. In contrast, platelet numbers decreased after irradiation alone to 125 ± 86 x 10 3 cells/μl. However, platelet numbers decreased even further after combined injury to 41 ± 15 x 10 3 cells/μl (Figure 6 A). sequence 1 or remaining netWebHistone H2AX Antibody [FITC] Summary Immunogen The immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for … séquence aire cmsequence acide sulfuriqueWebH2AFX / H2AX H2A histone family, member X. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA … séquence album ms